Vemurafenib, a BRAF V600E inhibitor, provides therapeutic benefits for patients with melanoma, but Padded Crank Bridle the frequent emergence of drug resistance remains a challenge.An understanding of the mechanisms underlying vemurafenib resistance may generate novel therapeutic strategies for patients with melanoma.Here, we showed that eIF3a, a translational regulatory protein, was an important mediator involved in vemurafenib resistance.eIF3a was expressed at significantly lower levels in vemurafenib-resistant A375 melanoma cells (A375R) than in parental A375 cells.Overexpression of eIF3a enhanced the sensitivity to BRAF inhibitors by reducing p-ERK Rangemaster lkd110dffsl Leckford Deluxe 110cm Dual Fuel Range Cooker levels.
Furthermore, eIF3a controlled ERK activity by regulating the expression of the phosphatase PPP2R1B via a translation mechanism, thus determining the sensitivity of melanoma cells to vemurafenib.In addition, a positive correlation between eIF3a and PPP2R1B expression was also observed in tumor samples from the Human Protein Atlas and TCGA databases.In conclusion, our studies reveal a previously unknown molecular mechanism of BRAF inhibitor resistance, which may provide a new strategy for predicting vemurafenib responses in clinical treatment.